DSS로 유도한 마우스의 대장염에 대한 돌배 추출물의 개선효과

• Published in: 농업생명환경연구, 33(0) pp. 166 - 175
• Main Authors: 이미라, 김보혜, 김남국, 김희정, 라문진, 정봉환, 이용준
• Format: Journal Article
• Language: Korean
• Published: 농업생명과학연구원 01.10.2021
• Subjects: 농학
• ISSN: 2233-8322; 2508-870X
• DOI: 10.22698/jales.20210017

Pyrus pyrifolia (Burm.f) Nakai fruit has been used as a traditional medicine for fever, cough, asthma, and rheumatic arthritis. This study aimed to investigate the anti-inflammatory effects of P. pyrifolia Nakai fruit extract (PPE) against DSS-induced colitis in mice. PPE—water extract (PWE), ethanol extract (PEE), or fermented extract (PFE)—was administered for 30 days. After 21 days of PPE treatment, ulcerative colitis was induced by oral administration of 2.5% DSS for 5 days. The mice were divided into seven groups: one normal control (NC) group and six groups with different treatments—DSS, DSS + 100 mg/kg PWE (PWL), DSS + 400 mg/kg PWE (PWH), DSS + 100 mg/kg PEE (PEL), DSS + 400 mg/kg PEE (PEH), DSS+ PFE (1:4 dilution, PFE), respectively. Mice treated with DSS showed severe bloody stools, diarrhea, and weight loss. Colon tissue in DSS group exhibited a marked decrease in its length. However, PPE treatment improved the disease activity index score and colon length. PPE alleviated DSS-induced inflammatory colon lesions. The level of IL-1β, a proinflammatory cytokine, was significantly decreased in PFE group. Moreover, colonic myeloperoxidase activity was significantly reduced in all PPE-treated groups, except in PWL group. These results show that PPE can be used for developing effective and safe functional health products for inflammatory bowel disease. KCI Citation Count: 1