Acinetobacter baumannii와 Candida albicans의 배양시간에 따른 자가형광발현과 pH의 변화 비교

• Published in: 한국임상치위생학회지, 9(1) pp. 11 - 19
• Main Authors: 이윤선, 한선영
• Format: Journal Article
• Language: Korean
• Published: 한국치위생감염관리학회 01.03.2021
• Subjects: 치의학
• ISSN: 2287-9641; 2465-7743
• DOI: 10.12972/kjcdh.20210002

Objectives: Quantitative Light-induced Fluorescence-Digital (QLF-D) was used to investigate the relationship between the autofluorescence characteristics of Acinetobacter baumannii and Candida albicans (a major causative agent and a risk factor for nosocomial pneumonia, respectively) and pH change. Methods: A. baumannii and C. albicans were the experimental microorganisms, and Streptococcus mutans was the control. Autofluorescence expression was assessed by smearing the cells on a solid medium and imaging for QLF-D at 48 h intervals for 168 h. The R/G ratio was calculated for the three types of colonies. The pH of the cultures (in liquid media) was measured for each incubation time. For statistical analysis, the Kruskal-Wallis test and Pearson correlation analysis were performed using SPSS version 25.0. Results: A. baumannii emitted orange fluorescence; the R/G ratio and pH increased at 24 h and peaked at 168 h, and exhibited positive correlation. C. albicans emitted reddish fluorescence; the R/G ratio and pH increased at 24 h, peaked at 120 h, and subsequently decreased. S. mutans did not exhibit fluorescence. Conclusions: The fluorescence of the A. baumannii cells was clearly observed, and the pH of the medium increased. The pH of the C. albicans culture peaked at 120 h and then decreased gradually. The correlation between autofluorescence and pH was confirmed. KCI Citation Count: 0