Isolation of Protease-producing Yeast, Pichia farinosa CO-2 and Characterization of Its Extracellular Enzyme
A yeast strain, CO-2, was isolated as a producer of extracellular protease from a sample of bamboo by-product. The cells were spherical- to ovoid-shaped and 3.7-4.2×4.1-4.8 µm in size. The isolated strain grew at 20-45℃, in the pH range of 3.0-12.0. Optimal growth conditions were 35℃ and pH of 6.0 f...
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| Published in | Applied biological chemistry pp. 133 - 141 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
한국응용생명화학회
01.04.2010
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| Subjects | |
| Online Access | Get full text |
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| Summary: | A yeast strain, CO-2, was isolated as a producer of extracellular protease from a sample of bamboo by-product. The cells were spherical- to ovoid-shaped and 3.7-4.2×4.1-4.8 µm in size. The isolated strain grew at 20-45℃, in the pH range of 3.0-12.0. Optimal growth conditions were 35℃ and pH of 6.0 for 14 h. The isolate was able to grow in up to 8.0%(w/v) NaCl and 9.0%(v/v) ethanol. Biochemical data obtained using the VITEK system were close to those of Pichia farinosa (exhibiting a 97% matching level). Based on molecular phylogenetic analysis of the 18S rDNA sequences and restriction enzyme analysis of the 18S rDNA-ITS1 region, this strain was identified as P. farinosa. Optimum pH and temperature of the crude protease preparation of P. farinosa CO-2 were pH 3.0 and 40℃, respectively. The enzyme retained more than 80% of its stability at pH ranging from 3.0 to 8.0. After heating at 60 C for 1 h, the enzyme exhibited 71% of its original activity. KCI Citation Count: 3 |
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| Bibliography: | G704-000111.2010.53.2.007 |
| ISSN: | 2468-0834 2468-0842 |