Enhanced saccharification of reed and rice straws by the addition of b-1,3-1,4-glucanase with broad substrate specificity and calcium ion

• Published in: Applied biological chemistry pp. 29 - 33
• Main Authors: 김동욱, 김희정, 정유석, 나한별, 차영록, 구본철, 김정호, 윤한대, 이정걸, 김훈
• Format: Journal Article
• Language: English
• Published: 한국응용생명화학회 01.02.2015
• Subjects: 농학
• ISSN: 2468-0834; 2468-0842

The possibility of using additive enzymes toimprove the saccharification of lignocellulosic substrateswith commercial cellulolytic enzymes was studied. Reed(Phragmites communis) and rice (Oryza sativa) strawpowders were pretreated with NaOH/steam via a hightemperatureexplosion system. The saccharification ofuntreated reed and rice straw powders by commercialenzymes (Celluclast 1.5 L ? Novozym 188) was not significantlyincreased by the addition of xylanases (Xyn10J,XynX), a cellulase (Cel6H), and a b-1,3-1,4-glucanase(BGlc8H) with broad substrate specificity. The saccharificationof the pretreated reed and rice straw powders by thecommercial enzymes was increased by 10.4 and 4.8 %,respectively, by the addition of BGlc8H. In the presence ofCa2? and BGlc8H, the saccharification of the pretreatedreed and rice straw powders by the commercial enzymeswas increased by 18.5 and 11.7 %, respectively. No sucheffect of Ca2? was observed with Xyn10J, XynX, orCel6H. The results suggest that the enzymatic conversionof lignocellulosic biomass to reducing sugars could beenhanced by certain additive enzymes such as b-1,3-1,4-glucanase, and that the enhancement could further beincreased by Ca2?. KCI Citation Count: 7