유방암에서 HER2 유전자 검사를 위한 자동화 은제자리부합법 검사의 유용성: 형광제자리부합법과 면역조직화학염색과의 비교

• Published in: Journal of pathology and translational medicine pp. 28 - 34
• Main Authors: 배영경, 성우정, 박석주, 구미진
• Format: Journal Article
• Language: Korean
• Published: 대한병리학회 01.02.2010
• Subjects: 병리학
• ISSN: 2383-7837; 2383-7845

Background : The human epidermal growth factor receptor 2 (HER2) is amplified in 20-25% of breast cancers. HER2 overexpression or amplification is associated with a worse clinical outcome and it can predict the benefit from anthracycline and anti-HER2 therapies. The HER2 status has usually been assessed by immunohistochemistry (IHC) or fluorescence in situ hybridization (FISH) in clinical samples. A new silver-enhanced in situ hybridization (SISH) technique was recently introduced. Therefore we evaluated the usefulness of SISH for detecting HER2 amplification. Methods : Tissue microarrays (TMAs) were constructed with 144 invasive breast cancer tissue samples. We performed IHC, FISH and SISH for HER2 on the tissue sections from the TMAs and we interpreted the results according to the American Society of Clinical Oncology/College of American Pathologists (ASCO/CAP) guidelines. The concordant rates between the two different tests were calculated. Results : HER2 was overexpressed and amplified in 16.9%, 16.9%, and 18% of the cases by IHC, FISH and SISH, respectively. The concordant rates between IHC and FISH, IHC and SISH, and FISH and SISH were 95.1%, 95.7%, and 97.8%, respectively. Conclusions : SISH can be an alternative test for evaluating HER2 amplification because the 97.8% concordance with FISH satisfies the ASCO/ CAP requirement of > 95% concordance with an alternative validated method. KCI Citation Count: 6