Next-Generation Sequencing-Based Transcriptome Analysis of L-Lysine-Producing Corynebacterium glutamicum ATCC 21300Strain

• Published in: The journal of microbiology pp. 877 - 880
• Main Authors: 김홍일, 남재영, 조재용, 이창수, 박영진
• Format: Journal Article
• Language: English
• Published: 한국미생물학회 01.12.2013
• Subjects: 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-013-3236-0

In the present study, 151 genes showed a significant change in their expression levels in Corynebacterium glutamicum ATCC 21300 compared with those of C. glutamicum ATCC 13032. Of these 151 genes, 56 genes (2%) were up-regulated and 95 genes (3%) were down-regulated. RNA sequencing analysis also revealed that 11 genes, involved in the L-lysine biosynthetic pathway of C. glutamicum, were up- or downregulated compared with those of C. glutamicum ATCC 13032. Of the 151 genes, 10 genes were identified to have mutations including SNP (9 genes) and InDel (1 gene). This information will be useful for genome breeding of C. glutamicum to develop an industrial amino acid-producing strain with minimal mutation. KCI Citation Count: 5