Preliminary Study about Sublingual Administration of Bacteriaexpressed Pandemic H1N1 Influenza Vaccine in Miniature Pigs

• Published in: The journal of microbiology pp. 794 - 800
• Main Authors: 김혜권, 김정기, 송호영, 최정아(국제백신연구소, 심병식, 강보규(녹십자수의약품, 문형준(녹십자수의약품, 염민주, 김상현, 송대섭, 송만기(국제백신연구소
• Format: Journal Article
• Language: English
• Published: 한국미생물학회 01.09.2014
• Subjects: 생물학
• ISSN: 1225-8873; 1976-3794
• DOI: 10.1007/s12275-014-4289-4

Sublingual (SL) administration of influenza vaccine would benon-invasive and effective way to give human populationsprotective immunity against the virus, especially when pandemicinfluenza outbreaks. In this study, the efficacy of pandemicinfluenza virus-based subunit vaccines was tested aftersublingual (SL) adjuvant administration in pigs. Eight specificpathogen-free Yucatan pigs were divided into 4 groups: nonvaccinatedbut challenged (A) and vaccinated and challenged(B, C, and D). The vaccinated groups were subdivided by vaccinetype and inoculation route: SL subunit vaccine (hemagglutininantigen 1 [HA1] + wild-type cholera toxin [wtCT],B); IM subunit vaccine (HA1 + aluminum hydroxide, C); andIM inactivated vaccine (+ aluminum hydroxide, D). The vaccineswere administered twice at a 2-week interval. All pigswere challenged with pandemic influenza virus (A/swine/GCVP-KS01/2009 [H1N1]) and monitored for clinical signs,serology, viral shedding, and histopathology. After vaccination,hemagglutination inhibition titre was higher in groupD (320) than in the other vaccinated groups (40–80) at thetime of challenge. The mobility and feed intake were reducedin group C. Both viral shedding and histopathological lesionswere reduced in groups B and D. Although this study haslimitation due to the limited number of pigs (2 pigs per agroup), the preliminary data in this study provided the protectivepotential of SL administration of bacteria-expressedpandemic H1N1 influenza vaccine in pigs. There should beadditional animal studies about effective adjuvant systemand vaccine types for the use of SL influenza vaccination. KCI Citation Count: 2