Characteristics of the Lipase from Candida rugosa Modified with Copolymers of Polyoxyethylene Derivative and Maleic Acid Anhydride

• Published in: The Korean journal of chemical engineering pp. 412 - 417
• Main Authors: 박진원, 박귀남, 민병렬, Honghyun Kim, Sanjeev Maken, Yeounsoo Kim
• Format: Journal Article
• Language: Korean
• Published: 한국화학공학회 01.05.2005
• Subjects: 화학공학
• ISSN: 0256-1115; 1975-7220

The hydrophilic copolymer, polyethylene oxide (PEO) allyl ester-maleic anhydride (MA), copolymer was used to modify the lipase from Candida rugosa. MA, in a functional group, reacts easily with amino acids of lipase.The degree of modification(DM) was varied by changing the weight ratio of copolymer to protein of lipase over therange of 10-120 (w/w). The specific activity decreased as DM increased. At the maximum modification degree of 35% ,the modified lipase retained more than 65% of the unmodified native lipase activity. The modified lipase displayed ahigh stability of activity against temperature and pH. The remaining activity of modified lipase was about 2-4 fold of% greater reaction of substrateat 10 hr than in the case where native lipase was used. KCI Citation Count: 7