Arabidopsis AMY1 expressions and early flowering mutant phenotype

• Published in: Journal of biochemistry and molecular biology pp. 101 - 105
• Main Authors: Wang Jie, Yu Dashi, Guo XinHong, Liu Xuanming
• Format: Journal Article
• Language: English
• Published: 한국생화학분자생물학회 01.02.2009
• Subjects: 생물학
• ISSN: 1225-8687; 0219-1024

The homozygous T-DNA mutant of the AMY1 gene in Arabidopsis was identified and importantly, shown to cause an early flowering phenotype. We found that the disruption of AMY1 enhanced expression of CO and FT. The expression analyses of genes related to starch metabolism revealed that expression of the AGPase small subunit APS1 in the wild type was higher than in the amy1 mutant. However, there were no significant differences in expression levels of the AGPase large subunit genes ApL1, AMY2, or AMY3 between wild type and the amy1 mutant. Expression profiling showed that AMY1 was highly expressed in leaves, stems, and flowers, and expressed less in leafstalks and roots. Furthermore, the level of AMY1 mRNA was highly elevated with age and in senescing leaves. RT-PCR analyses showed that the expression of AMY1 was induced by heat shock, GA, and ABA, while salt stress had no apparent effect on its expression. [BMB reports 2009; 42(2): 101-105] KCI Citation Count: 1