Crossbreeding of cry1Ac/bar Transgenic Progeny with a Non-transgenic Elite Rice Cultivar for Minimizing the Underlying Grain Cost of Bt Transgenic Rice

• Published in: Plant breeding and biotechnology pp. 21 - 29
• Main Authors: 우미옥, 이진형, 신성휴, 신강식, 고희종, 서석철
• Format: Journal Article
• Language: English
• Published: 한국육종학회 01.03.2015
• Subjects: 농학
• ISSN: 2287-9358; 2287-9366

Most Bt transgenic rice events have an underlying grain cost depending on the insect pressure in the field. This studywas conducted (1) to determine if the cry1Ac/bar genetically modified (GM) rice (designated Agb0101) based on a Korean japonicarice cultivar has any underlying grain cost and (2) to develop cry1Ac/bar GM rice lines with yield performance similar to those ofKorean elite cultivars via crossbreeding. A three-year field experiment showed that Agb0101 had a high underlying grain costequivalent to 18% grain yield loss, and this cost was mainly due to the grain number per panicle. Moreover, it was found that thecry1Ac/bar transgene was inherited as a single dominant gene. BC1F4―F5 crossbred progeny derived from an anther culture ofAgb0101―showed phenotypic resemblance to non-transgenic Korean elite cultivars, resulting in a decrease in the grain yield cost ofAgb0101 from 27% to 10%. These results suggest that Bt-GM rice-oriented cross-fertilization with a non-GM elite cultivar followed bytwo- or threefold selection is a feasible method to minimize or remove the underlying grain cost of the Bt transgenic insect-resistant riceline. KCI Citation Count: 0