The Ribostamycin Biosynthetic Gene Cluster in Streptomyces ribosidificus: Comparison with Butirosin Biosynthesis

A cluster of genes for ribostamycin (Rbm) biosynthesis was isolated from Streptomyces ribosidificus ATCC 21294. Sequencing of 31.892 kb of the genomic DNA of S. ribosidificus revealed 26 open reading frames (ORFs) encoding putative Rbm biosynthetic genes as well as resistance and other genes. One of...

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Bibliographic Details
Published inMolecules and cells pp. 90 - 96
Main Authors 송재경, Bimala Subba, Madan Kumar Kharel, Hei Chan Lee, 류광경, 김병기
Format Journal Article
LanguageKorean
Published 한국분자세포생물학회 01.08.2005
Subjects
생물학
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ISSN1016-8478
0219-1032

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Summary:A cluster of genes for ribostamycin (Rbm) biosynthesis was isolated from Streptomyces ribosidificus ATCC 21294. Sequencing of 31.892 kb of the genomic DNA of S. ribosidificus revealed 26 open reading frames (ORFs) encoding putative Rbm biosynthetic genes as well as resistance and other genes. One of ten putative Rbm biosynthetic genes, rbmA, was expressed in S. lividans TK24, and shown to encode 2-deoxy-scyllo-inosose (DOI) synthase. Acetylation of various aminoglycosideaminocyclitol (AmAcs) by RbmI confirmed it to be an aminoglycoside 3-N-acetyltransferase. Comparison of the genetic control of ribostamycin and butirosin biosynthesis pointed to a common biosynthetic route for these compounds, despite the considerable differences between them in genetic organization. KCI Citation Count: 26
Bibliography:G704-000079.2005.20.1.017
ISSN:1016-8478
0219-1032