허혈 및 저산소 전조건화가 신생 백서의 저산소-허혈 뇌손상에 미치는 보호 효과: 수소 자기 공명 분광법을 이용한 연구

• Published in: Korean journal of anesthesiology pp. 188 - 197
• Main Authors: 정성문, 정화성, 최재문, 방지연, 임근호, 박평환
• Format: Journal Article
• Language: Korean
• Published: 대한마취통증의학회 01.02.2006
• Subjects: 마취과학
• ISSN: 2005-6419; 2005-7563

Background: A brief episode of cerebral ischemia confers transient ischemic tolerance to a subsequent ischemic challenge. We examined the effect of ischemic and hypoxic preconditioning in the neonatal rat. Methods: Seven-day old Sprague-Dawley rat pups were divided into three groups:control (n = 53), ischemic preconditioning (n = 51), and hypoxic preconditioning (n = 48). For ischemic preconditioning, the right common carotid artery was occluded for 10 min. Rats in the hypoxic preconditioning group were kept under hypoxic (8% oxygen/92% nitrogen) conditions for 4h. Twenty-four hours after the preconditioning, rats from all groups were exposed to the right common carotid artery ligature, followed by 2.5 h of hypoxia. Lipid/N-acetyl aspartate (Lip/NAA) and lipid/creatine (Lip/Cr) ratios from 1H MR spectroscopy and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) were evaluated as measures of apoptosis 1 and 7 days after hypoxic-ischemic injury. Results: In the ischemic and hypoxic preconditioning groups, the Lip/NAA and Lip/Cr ratios and the numbers of TUNEL- positive cells were significantly lower than those in the control group (P ＜ 0.05), but there were no significant differences between the two preconditioning groups. Conclusions: These results suggest that ischemic and hypoxic preconditioning in the neonatal rat attenuate the apoptosis that is caused by hypoxic-ischemic brain injury. (Korean J Anesthesiol 2006; 50: 188～97) KCI Citation Count: 0