Involvement of Corticotropin-releasing Factor Receptor 2beta in Differentiation of Dopaminergic MN9D Cells

• Published in: Molecules and cells pp. 243 - 249
• Main Authors: 진태은, 장미애, 김현정, 최유미, 정성권, 박명규
• Format: Journal Article
• Language: English
• Published: 한국분자세포생물학회 01.09.2008
• Subjects: 생물학
• ISSN: 1016-8478; 0219-1032

Corticotropin releasing factor (CRF) mediates various responses to stress through CRF receptors 1 and 2. CRF receptor 2 has two forms, 2alpha and 2beta each of which appears to have distinct roles. Here we used dopaminergic neuron-derived MN9D cells to investigate the function of CRF receptor 2 in dopamine neurons. We found that n-butyrate, a histone deacetylase inhibitor, induced MN9D cell differentiation and increased gene expression of all CRF receptors. CRF receptor 2beta was minimally expressed in MN9D cells; however, its expression dramatically increased during differentiation. CRF receptor 2beta expression levels appeared to correlate with neurite outgrowth, suggesting CRF receptor 2beta involvement in neuronal differentiation. To validate this statement, we made a CRF receptor 2beta-overexpressing MN9D/CRFR2 stable cell line. This cell line showed robust neurite outgrowth and GAP43 overexpression, together with MEK and ERK activation, suggesting MN9D cell neuronal differentiation. From these results, we conclude that CRF receptor 2beta plays an important role in MN9D cell differentiation by activating the MEK/ERK signaling pathway. KCI Citation Count: 1