The Expression Profile and Function of Satb2 in Zebrafish Embryonic Development

• Published in: Molecules and cells pp. 337 - 382
• Main Authors: 안현종, 박유진, 김수현, 박해철, Su-Kyoung Seo, 여상엽, 금동호
• Format: Journal Article
• Language: English
• Published: 한국분자세포생물학회 01.10.2010
• Subjects: 생물학
• ISSN: 1016-8478; 0219-1032

The present study shows the expression profile and func-tion of the homeobox gene, satb2 during zebrafish embry-onic development. Satb2 was ubiquitously expressed from the 1 cell stage to the 10-somite stage in zebrafish em-bryos. Satb2 showed stage-specific expression profiles such as in the pronephric duct at 24 hpf, the branchial arches at 36 hpf, and the ganglion cell layer of the retina and fins at 48 hpf. Additionally, satb2 knockdown embryos were arrested at 50-60% epiboly, and transplantation ex-periments with satb2 knockdown cells showed migration defects. Interestingly, satb2 knockdown cells also exhib-ited down-regulation of dynamin II and VAMP4, which are involved in exocytosis and endocytosis, respectively. Fur-thermore, satb2 knockdown cells have a disorganized actin distribution and an underdeveloped external yolk syncytial layer, both of which are involved in epiboly. These results suggest that satb2 has a functional role in epiboly. This role may potentially be the regulation of endo-exocytic vesicle transport-dependent cell migration and/or the regulation of the development of the yolk syncytial layer. KCI Citation Count: 11