액체 크로마토그라피에 의한 Human Neutrophil Granular Proteases의 분리

Human leukocyte elastases were purified by gel filtration and ion exchange-chromatography. For gel filtration step, Ultrogel AcA 54 gave extremly good resolution and separation for highly positive charged proteins. Ultrogel AcA 54 also showed excellent separation characteristics of two different siz...

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Bibliographic Details
Published inKosin Medical Journal (Online) pp. 9 - 14
Main Authors 정혜영, 김사열(Ghim Sa-Youl), 강구일
Format Journal Article
LanguageKorean
Published 고신대학교 의과대학 학술지 01.03.1987
Subjects
의학일반
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Summary:Human leukocyte elastases were purified by gel filtration and ion exchange-chromatography. For gel filtration step, Ultrogel AcA 54 gave extremly good resolution and separation for highly positive charged proteins. Ultrogel AcA 54 also showed excellent separation characteristics of two different size of highly positive charged molecules like elastase and cathepsin G. Both CM-Cellulose and CM-Sephadex showed the possibility to separate the individual molecules of three isozymes. CM-Sephadex showed the better resolution for separation of individual isozyme without contamination of cathepsin G molecules. KCI Citation Count: 0
ISSN:2005-9531
2586-7024