말똥성게 (Hemicentrotus pulcherrimus)의 수정 및 배 발생률을 이용한 해양배출 폐기물의 독성평가

• Published in: 환경독성보건학회지, 25(1) pp. 11 - 18
• Main Authors: 황운기, 류향미, 김성길, 박종수, 안경호
• Format: Journal Article
• Language: Korean
• Published: 환경독성보건학회 01.03.2010
• Subjects: 기타의약학
• ISSN: 2093-6400

Toxicity assessment of ocean dumping wastes (dye waste, urban sewage, food waste) were examined in the fertilization and embryo development rates of the Sea Urchin, Hemicentrotus pulcherrimus. Spawning was induced by injecting 1 mL of 0.5 M KCl into coelomic cavity. Males released white or cream-colored sperms and females released yellow or orange-colored eggs. Experiments were began within 30 min the collection of both gametes. The fertilization and embryo development rates test were performed for 10 min and 64 h after fertilization, respectively. The fertilization and embryo development rates in the control condition (not including ocean dumping wastes sludge elutriate) were greater than 90%, but suddenly decreased with increasing of ocean dumping waste sludge elutriate concentrations. The fertilization and normal embryogenesis rates were significantly inhibited in all waste sludge elutriate from dye waste (EC50=4.37; EC50=1.76), urban sewage (EC50=5.79; EC50=2.00) and food waste (EC50=7.68; EC50=2.16), respectively. The NOEC (⁄3.13) and LOEC (3.13) of fertiliztion and normal embryogenesis rates very similar in all waste sludge elutriate. These results suggest that biological assay using the fertilization and embryo development rates of H. pulcherrimus are very useful test method for the ecological toxicity assessment of ocean dumping wastes. KCI Citation Count: 2