Stem Cells Expressing Homing Receptors Could be Expanded From Cryopreserved and Unselected Cord Blood

• Published in: Journal of Korean medical science pp. 635 - 639
• Main Authors: 이영호, Seo Su Yeong, Kim Kyeong Hee, Lee Young Ah, Lee Young Seok, Lee Hyung Sik, Hur Won Joo, Han Hun, Kwon Hyuk Chan, Kim Jae Seok, Kim HyoJin, Han Jin Yeong
• Format: Journal Article
• Language: Korean
• Published: 대한의학회 01.10.2004
• Subjects: 의학일반
• ISSN: 1011-8934; 1598-6357

We assessed the cytokine combinations that are best for ex vivo expansion of cord blood (CB) and the increment for cell numbers of nucleated cells, as well as stem cells expressing homing receptors, by an ex vivo expansion of cryopreserved and unselected CB. Frozen leukocyte concentrates (LC) from CB were thawed and cultured at a concentration of 1 ×10 5/mL in media supplemented with a combination of SCF (20 ng/mL)+TPO (50 ng/mL)+FL (50 ng/mL)±IL-6 (20 ng/mL)±G-CSF (20 ng/mL). After culturing for 14 days, the expansion folds of cell numbers were as follows: TNC 22.3 ±7.8~26.3 ±4.9, CFU-GM 4.7 ±5.1~11.7 ±2.6, CD34+CD38- cell 214.0 ±251.9~464.1 ±566.1, CD34+CXCR4+ cell 4384.5 ±1664.7~7087.2 ±4669.3, CD34+VLA4+ cell 1444.3 ±1264.0~2074.9 ±1537.0, CD34+VLA5+ cell 86.2 ±50.9~ 113.2 ±57.1. These results revealed that the number of stem cells expressing hom-ing receptors could be increased by an ex vivo expansion of cryopreserved and unselected CB using 3 cytokines (SCF, TPO, FL) only. Further in vivo studies regarding the engraftment after expansion of the nucleated cells, as well as the stem cells expressing homing receptors will be required. KCI Citation Count: 0