The Effect of Each rhBMP2 from CHO and E.Coli Coated Bone Grafting Materials in New Bone Formation

• Published in: Biomaterials research pp. 119 - 124
• Main Authors: 이광일, 이근수, 최욘락, 김영식, 정홍희, 김영우, 강영택, 문성환, 심영복, 장주웅
• Format: Journal Article
• Language: English
• Published: 한국생체재료학회 01.09.2011
• Subjects: 의공학
• ISSN: 1226-4601; 2055-7124

Bone grafting materials, those are hydroxyapatite coated with beta tricalcium phosphate (β-TCP), cortical bone, cancellous bone and cortico-cancellous bone powder, were separately coated with two types of recombinant human bone morphogenetic protein 2 (rhBMP2) by freeze drying. Each rhBMP2 was expressed from different hosts of Escherichia coli (E.Coli) and Chinese hamster ovary (CHO) cell. Each grafting material was implanted into dorsal muscle pouch of nude mouse for 3 and 6 weeks. At 3 and 6 weeks after implantation, X-ray for radiological observation,component analysis for Ca/P ratio, and histological evaluation for osteoinductivity were processed. At 3-week post-op, CHO cell expressed BMP2 treated grafting materials were earlier bone absorption than other groups. Specially,the bone absorption of cancellous bone was significantly affected with CHO expressed BMP2 at 3-week point. In case of 6-week post-op, the group of CHO cell expressed BMP2 showed highly new bone formation and also the control group which was not treated with BMP2 showed higher osteoinductivity than the group of E.Coli expressed BMP2. It didn't have any correlation with new bone formation by human bone powders. However, various human bone powders were good materials for bone grafting and the combination of CHO expressed BMP2significantly had synergy effect for new bone formation. KCI Citation Count: 0