염증 및 분화 관련 세포 반응에 대한 치과용 Mineral Trioxide Aggregate의 영향

• Published in: Biomaterials research pp. 7 - 12
• Main Authors: 김종길, 손경미, 박희철, 주정정, 권지현, 양형철
• Format: Journal Article
• Language: Korean
• Published: 한국생체재료학회 01.03.2013
• Subjects: 의공학
• ISSN: 1226-4601; 2055-7124

Mineral trioxide aggregate (MTA) is receiving more attentions in endodontic dental clinics due to its high sealing ability and biocompatibility. Although previous studies have reported the effects of MTA on dental pulp tissue, the mechanisms underlying on inflammation and differentiation at cellular levels are not yet fully understood. In this study, we investigated various molecular aspects of cellular reactions involved in inflammation and differentiation to commercially available MTAs including ProRoot MTA, Ortho MTA and MTA-Angelus. mRNA of COX-2 gene which is involved in inflammatory reaction was not increased by all tested MTAs, and expression of COX-2 by lipopolysaccharide was not severely altered by MTAs, demonstrating that COX-2 is not involved in inflammatory reactions to MTA. The extracts of all MTAs obtained for early 24 hrs increased ALP activities of human dental pulp (HDP) cells, while the extracts of ProRoot MTA and MTA-Angelus obtained for 1-3 and 3-6 day decreased ALP activity, indicating the presence of inhibitory components in the two MTAs. ProRoot MTA enhanced mRNA levels of DMP-1, but not DSPP. However, the other MTAs did not affect mRNA expression of DSPP, DMP-1, OCN and OPN, which is not consistent with ALP activities. Thus, further studies are needed to conclude the effects of MTA on differentiation of dental pulp cells. KCI Citation Count: 0