夏枯草에서 추출한 Ursolic acid의 파골세포 분화 억제 효과

• Published in: 대한한방부인과학회지, 27(2) pp. 59 - 70
• Main Authors: 허자경, 황덕상, 이진무, 이창훈, 장준복, 이경섭
• Format: Journal Article
• Language: Korean
• Published: 대한한방부인과학회 01.05.2014
• Subjects: 한의학
• ISSN: 1229-4292; 2508-3619
• DOI: 10.15204/jkobgy.2014.27.2.059

Purpose: This study was conducted to evaluate the inhibitory effect of ursolicacid from Prunella vulgaris on osteoclast differentiation. Methods: MTT-assay was performed to estimate cytotoxicity of ursolic acidfrom Prunella vulgaris in BMMs stimulated with M-CSF. TRAP staining, TRAPactivity and Real-time PCR were performed to know the inhibitory effect onosteoclast differentiation. Actin ring formation were analysed to observe the effectof ursolic acid from Prunella vulgaris. Results: Ursolic acid from Prunella vulgaris has no cytotoxicity at the concentrationof 1 μg/ml or lower. Ursolic acid decreased the number of TRAP positive cells andthe expression of NFATc1 gene, c-Fos gene, TRAP and OSCAR in BMMs stimulatedwith RANKL. Ursolic acid restrained the formation of actin ring. Ursolic acidinhibited NF-κB activity by inducing degradation of p-IkBɑ. Conclusions: Ursolic acid from Prunella vulgaris has the inhibitory effect ofosteoclast differentiation and bone resorption. Futher studies are needed to treatosteoporosis by usolic acid from Prunella vulgaris. KCI Citation Count: 4