Diallyl Trisulfide Suppresses the Production of Lipopolysaccharide-induced Inflammatory Mediators in BV2 Microglia by Decreasing the NF-B Pathway Activity Associated With Toll-like Receptor 4 and CXCL12/CXCR4 Pathway Blockade

• Published in: Journal of cancer prevention pp. 134 - 140
• Main Authors: 이혜현, 정진우, 홍수현, 박철, 김병우, 최영현
• Format: Journal Article
• Language: English
• Published: 대한암예방학회 01.09.2018
• Subjects: 예방의학
• ISSN: 2288-3649; 2288-3657
• DOI: 10.15430/JCP.2018.23.3.134

Background: Diallyl trisulfide (DATS), a garlic-derived organosulfuric compound, has been documented for potential anti-inflammatoryeffects. However, the mechanism in microglia remains unknown. In this study, we investigated the anti-inflammatory effects of DATSin lipopolysaccharide (LPS)-stimulated BV2 microglial cells. Methods: The effects of DATS on LPS-induced pro-inflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2) wereassessed under conditions not in the cytotoxicity of DATS. The protein expression of inflammation regulatory genes was measured byWestern blot analysis. Results: DATS significantly inhibited the LPS-induced secretion of NO and PGE2, which was associated with the suppression of theirregulatory genes, inducible NO synthase and COX-2. DATS had been shown to inhibit nuclear translocation of NF-B by destroying thedegradation and phosphorylation of IB- inhibitors in the cytoplasm. In addition, DATS effectively inhibited the expression of LPS-inducedtoll-like receptor 4 (TLR4) and myeloid differentiation factor 88. Furthermore, DATS markedly reduced the LPS-induced expression ofchemokine (CXC motif) ligand (CXCL) 12 and CXC receptor (CXCR) 4, demonstrating its capacity to block chemo-attractive activity. Conclusions: These results indicate that DATS inhibits the activation of the CXCL12/CXCR4 axis associated with antagonizing effect onTLR4 and blocks NF-B signaling, thus demonstrating anti-inflammatory effects against LPS stimulation. KCI Citation Count: 0