두릅 아세트산 에틸 분획물의 산화방지 효과 및 알코올에 대한간세포 보호효과

• Published in: 한국식품과학회지, 50(2) pp. 216 - 224
• Main Authors: 권봉석(경상대학교 응용생명과학부, 박선경(경상대학교 응용생명과학부, 김종민(경상대학교 응용생명과학부, 강진용(경상대학교 응용생명과학부, 박상현(경상대학교 응용생명과학부, 강정은(경상대학교 응용생명과학부, 이창준(경상대학교 응용생명과학부, 박수빈(경상대학교 응용생명과학부, 유슬기(경상대학교 응용생명과학부, 이욱, 허호진
• Format: Journal Article
• Language: Korean
• Published: 한국식품과학회 01.04.2018
• Subjects: 식품과학
• ISSN: 0367-6293
• DOI: 10.9721/KJFST.2018.50.2.216

To evaluate physiological effect of Aralia elata, in vitro antioxidant activity and hepatic protective effects were investigated. Ethyl acetate fraction from Aralia elata (EFAE) had higher total phenolic content than other fractions (nhexane, chloroform, and distilled water layers). EFAE also showed significantly greater radical scavenging activity against 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH), than other fractions. Moreover, EFAE showed dose-dependent inhibitory effect of malondialdehyde (MDA). Hepatoprotective effects of EFAE against ethanol- and H2O2-induced oxidative stress and cytotoxicity in H4IIE and HepG2 hepatic cells were examined using 2',7'-dichlorofluorescein diacetate (DCF-DA) and 3-[4,5-dimethythiazol-2-yl]-2,5- diphenyl tetrazolium bromide (MTT) assay. The results showed that EFAE reduced cellular oxidative stress, and increased hepatic cell viability. In addition, EFAE inhibited ethanol-induced lipid accumulation in HepG2 cells. Finally, physiological substances of EFAE were analyzed using high performance liquid chromatography (HPLC), and the major bioactive compounds identified were 3,5-dicaffeoylquinic acid and chlorogenic acid. KCI Citation Count: 1