New Method for Simultaneous Quantifi cation of 12 Ginsenosides in Red Ginseng Powder and Extract: In-house Method Validation

• Published in: Journal of ginseng research pp. 205 - 210
• Main Authors: Gyo In, Nam-Geun Ahn, Bong-Seok Bae, Sung-Tai Han, Kil-Bong Noh, Cheon-Suk Kim
• Format: Journal Article
• Language: English
• Published: 고려인삼학회 01.04.2012
• Subjects: 기타의약학
• ISSN: 1226-8453; 2093-4947
• DOI: 10.5142/jgr.2012.36.2.205

For quality control of components in Korean red ginseng powder and extract, a new method for simultaneous quantifi cation of 12 ginsenosides (Rg1, Re, Rf, Rh1, Rg2[S], Rg2[R], Rb1, Rc, Rb2, Rd, Rg3[S], and Rg3[R]) was studied. Compared to the offi cial method for quantifi cation of marker substances (ginsenosides Rg1 and Rb1), the proposed methods were guaranteed by in-house method validation. Several criteria such as linearity, specifi city, precision and accuracy were evaluated. For red ginseng powder,recovery (averaging 95% to 105%) was calculated, and analysis of variance was carried out to estimate the relative standard deviation (0.20% to 2.12%). For red ginseng extract, the average recovery rate was 90% to 99% and the relative standard deviation was 0.39% to 2.40%. These results indicate that the proposed method could be used in the laboratory for determination of 12ginsenosides in red ginseng powder and extract. In addition, this method was found to be suitable for quality control of ginseng products and potentially offer time and cost benefits. KCI Citation Count: 18