B6C3F1 mice exposed to ozone with 4-(N-methy1-N-nitrosamino)-1-(3-pyridy1)-1-butanone and/or dibuty1 phthalate showed toxicities through alterations of NF-kB, AP-1,Nrf2, and osteopontin

• Published in: Journal of veterinary science (Suwŏn-si, Korea) pp. 131 - 137
• Main Authors: 조명행, 김민영, 송경석, 박건호, 장승희, 김현우, 박진홍, 진화, 유국종, 조현선, 강가미, 김영철
• Format: Journal Article
• Language: English
• Published: 대한수의학회 01.06.2004
• Subjects: 수의학
• ISSN: 1229-845X; 1976-555X

Toxic effects of ozone, 4-(N-methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK), and/or dibutyl phthalate (DBP)were examined through NF-κB, AP-1, Nrf2, and osteopontin (OPN) in lungs and livers of B6C3F1 mice. Electrophoretic mobility shift assay (EMSA) indicated that mice treated with combination of toxicants induced high NF-κB activities. Expression levels of p105, p65, and p50 proteins increased in all treated mice, whereas IκB activity was inhibited in NNK-,DBP-, and combination-treated ones. All treated mice exceptozone-treated one showed high AP-1 binding activities. Expression levels of c-fos, c-jun, junB, jun D, Nrf2, and OPNproteins increased in all treated mice. Additive interactions were frequently noted from two-toxicant combination micecompared to ozone-treated one. These results indicate treatment of mixture of toxicants increased toxicity through NF-κB, AP-1, Nrf2, and OPN. Our data could be applied to the elucidation of mechanism as well as the risk assessment of mixture-induced toxicity. KCI Citation Count: 0