Molecular Characterization of Apple stem grooving virus Isolated fromTalaromyces flavus

• Published in: The plant pathology journal pp. 260 - 264
• Main Authors: 심혜경, 심창기, 지형진, 이석찬, Kyu-Hyon Hwang, Su-Wan Son, 김동균, Yong-Mun Choi, 정영재, Dae-Hyun Kim
• Format: Journal Article
• Language: English
• Published: 한국식물병리학회 01.09.2006
• Subjects: 농학
• ISSN: 1598-2254; 2093-9280

Talaromyces flavus mediates the transmission of Applestem grooving virus (ASGV) to several host plants. TheASGV-F carried by T. flavus was partially purified fromthe fungus. Based on sequence analysis and homologysearches, this is closely related to other ASGV strainsisolated from host plants. The partially purified viralcoat protein (CP) was separated on a 12% SDSpolyacrylamidegel and analyzed by Western blottingwith an ASGV anti-serum. A single band at 28 kDareacted with the ASGV anti-serum. The deduced aminoacid sequence of the ORF-1 showed conserved domains,including an NTP-binding helicase motif, GFAGSGKT. The amino acid sequences of the helicase and CPshowed strong homology to other ASGV strains (98%). All ASGV isolated from plants and fungi had saltbridges composed of the CP and the GFAGSGKT motifof the helicase, which are commonly conserved in plantviruses. These results suggest that ASGV-F is one ofASGV strains isolated from T. flavus based on sequencesimilarity as well as the serological analysis of CP. Molecular Characterization of Apple stem grTalaromyces flavus mediates the transmission of Applestem grooving virus (ASGV) to several host plants. TheASGV-F carried by T. flavus was partially purified fromthe fungus. Based on sequence analysis and homologysearches, this is closely related to other ASGV strainsisolated from host plants. The partially purified viralcoat protein (CP) was separated on a 12% SDSpolyacrylamidegel and analyzed by Western blottingwith an ASGV anti-serum. A single band at 28 kDareacted with the ASGV anti-serum. The deduced aminoacid sequence of the ORF-1 showed conserved domains,including an NTP-binding helicase motif, GFAGSGKT. The amino acid sequences of the helicase and CPshowed strong homology to other ASGV strains (98%). All ASGV isolated from plants and fungi had saltbridges composed of the CP and the GFAGSGKT motifof the helicase, which are commonly conserved in plantviruses. These results suggest that ASGV-F is one ofASGV strains isolated from T. flavus based on sequencesimilarity as well as the serological analysis of CP.ooving virus Isolated fromTalaromyces flavus KCI Citation Count: 2