Expression of Cell Surface Receptors on Human Glioblastoma Xenograft Model in NOD/SCID Mouse

• Published in: Cancer research and treatment pp. 52 - 57
• Main Authors: Kyung Seung Oh, Ki Uk Kim, Na Hee Park, Su Yeong Seo, Sun Seob Choi, Gi Yeong Huh
• Format: Journal Article
• Language: Korean
• Published: 대한암학회 01.02.2002
• Subjects: 의약학
• ISSN: 1598-2998; 2005-9256

Purpose: To obtain basic data for development of a glioblastoma-specific immunotoxin, the expression of variable cell surface receptors on a human glioblastoma xenograft model was evaluated, using NOD/SCID mice. Materials and Methods: We developed a xenograft model in NOD/SCID mice implanted with a human glioblastoma cell line (U-87MG). Immunohistochemical studies were performed on implanted tumor nodules (n=8) using antibodies against CD71, EGFR, IGF-IRα, CXCR4 and IL-4Rα. Results: Expression of IL-4Rα, in implanted tumor nodules, was the highest of the cell surface receptors evaluated in this study. However, the endothelial cells in, and around, the tumor nodules also revealed immunopositivity against IL-4Rα. The immunoreactivity of IL-4R α, and other surface receptors such as CD71, IGF-IRα and EGFR, was prominent in tumor nodules associated with tumor necrosis. Conclusion: IL-4Rα would be a possible target for the development of glioblastoma-specific immunotoxin, although there are limitations due to its endothelial expression. (Cancer Res Treat. 2002;34:52-57) KCI Citation Count: 0