흰쥐 음경해면체신경 손상 모델에서 제대혈 유래 인간 중간엽 줄기세포 치료의 효과

• Published in: The world journal of men's health pp. 136 - 141
• Main Authors: 최아람, 김성대, 손동완, 김두배, 김현우, 조수연, 조용현, 김세웅
• Format: Journal Article
• Language: Korean
• Published: 대한남성과학회 01.09.2008
• Subjects: 비뇨기과학
• ISSN: 2287-4208; 2287-4690

Purpose: To find out the effect of transplanted umbilical cord blood derived human mesenchymal stem cells (hUCB- MSCs) in the rat model of bilateral cavernosal nerve injury (BCNX) Materials and methods: Male Sprague Dawley rats were divided into three groups: Group I (7 rats, Control, Sham operation) had only bilateral cavernosal nerve crushing injury. PKH-26 fluorescent cell linker was labeled to hUCB- MSCs Group II (7 rats) had transplantation of hUCB- MSCs (3×105cells/50μl PBS) into MPG after BCNX. Group III (7 rats) had transplantation of hUCB- MSCs (3×105cells/50μl PBS) into the CC after BCNX. We detected the PKH-26 labeled MSCs in Group II, and III at four weeks after transplantation. Peak intracavernosal pressure (ICP) and ICP/mean arterial pressure (MAP) were recorded after pelvic nerve stimulation at 12 weeks after transplantation. Results: PKH-26 labeled hUCB-MSCs were detected in MPG and CC of group II and III at four weeks after transplantation. The peak ICP was significantly higher in the Group II (69.6±5.9mmHg) and Group III (59.7±7.6mmHg) compared to Group I40.6±10.5mmHg) at 12 weeks after transplantation. The rates of ICP/MAP were significantly higher in Group II (32.2±6.4%) and Group III (57.8±4.9%) compared to Group I (49.5±2.9%) at 12 weeks after transplantation. Conclusions: The intracavernosal and major pelvic ganglion transplantation of hUCB- MSCs improve erectile function in a rat model of neurogenic impotence. Purpose: To find out the effect of transplanted umbilical cord blood derived human mesenchymal stem cells (hUCB- MSCs) in the rat model of bilateral cavernosal nerve injury (BCNX) Materials and methods: Male Sprague Dawley rats were divided into three groups: Group I (7 rats, Control, Sham operation) had only bilateral cavernosal nerve crushing injury. PKH-26 fluorescent cell linker was labeled to hUCB- MSCs Group II (7 rats) had transplantation of hUCB- MSCs (3×105cells/50μl PBS) into MPG after BCNX. Group III (7 rats) had transplantation of hUCB- MSCs (3×105cells/50μl PBS) into the CC after BCNX. We detected the PKH-26 labeled MSCs in Group II, and III at four weeks after transplantation. Peak intracavernosal pressure (ICP) and ICP/mean arterial pressure (MAP) were recorded after pelvic nerve stimulation at 12 weeks after transplantation. Results: PKH-26 labeled hUCB-MSCs were detected in MPG and CC of group II and III at four weeks after transplantation. The peak ICP was significantly higher in the Group II (69.6±5.9mmHg) and Group III (59.7±7.6mmHg) compared to Group I40.6±10.5mmHg) at 12 weeks after transplantation. The rates of ICP/MAP were significantly higher in Group II (32.2±6.4%) and Group III (57.8±4.9%) compared to Group I (49.5±2.9%) at 12 weeks after transplantation. Conclusions: The intracavernosal and major pelvic ganglion transplantation of hUCB- MSCs improve erectile function in a rat model of neurogenic impotence. KCI Citation Count: 2