Study of Thermostable Chitinase Enzymesfrom Indonesian Bacillus K29-14

• Published in: Journal of microbiology and biotechnology pp. 647 - 652
• Main Authors: SRI, RAHAYU(Bogor Agricultural University, Indonesia), FREDY TANUWIDJAJA,(Bogor Agricultural University, Indonesia), YAYA RUKAYADI,(Bogor Agricultural University, Indonesia), ANTONIUS SUWANTO(Bogor Agricultural University, Indonesia), MAGGY THENAWIDJAJA SUHARTONO(Bogor Agricultural University, Indonesia), JAE KWAN HWANG, YU RYANG PYUN
• Format: Journal Article
• Language: Korean
• Published: 한국미생물·생명공학회 01.08.2004
• Subjects: 생물학
• ISSN: 1017-7825

Thermophilic microorganisms capable of producingchitinase enzymes were screened from samples collectedfrom several crater and geothermal areas. The chitinolyticmicroorganisms were grown in a selective medium containingcolloidal chitin. The Bacillus K29-14 isolate was found toexhibit the highest chitinase and chitin deacetylase activities.When grown in a chitin-containing medium, the isolateproduced extracellular chitinase after 24 h of incubation. Theoptimum temperature and pH for the chitinase were 55oC andpH 7, respectively, while those for the chitin deacetylase were55oC and pH 8, respectively. The thermostable chitinase andchitin deacetylase also retained 80- 90% of their activity afterincubation for 5 h at 70oC. The divalent cations CoCl2 andNiCl2 increased the chitinase activity, while ZnCl2 inhibitedthe enzyme. The chitin deacetylase was also activated by thepresence of MgCl2 and inhibited by MnCl2, NiCl2, and CaCl2.A zymogram analysis revealed several forms of chitinase,with a 67 kDa form being the major enzyme. KCI Citation Count: 6