Isolation and Characterization of Ksugamycin Biosynthetic Genes from Streptomyces ksugaensis KACC 20262

The biosynthetic gene cluster for the aminoglycosideantibiotic kasugamycin was isolated and characterized fromthe kasugamycin producing strain, Streptomyces kasugaensisKACC 20262. By screening a fosmid library using kasA, thegene encoding aminotransferase, we isolated a 22 kb DNAfragment. The fragme...

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Published inJournal of microbiology and biotechnology pp. 491 - 496
Main Authors Jo, You-Young, Jing Liu, Ying-Yu Jin, Young-Yell Yang, Joo-Won Suh
Format Journal Article
LanguageKorean
Published 한국미생물·생명공학회 01.06.2005
Subjects
생물학
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Summary:The biosynthetic gene cluster for the aminoglycosideantibiotic kasugamycin was isolated and characterized fromthe kasugamycin producing strain, Streptomyces kasugaensisKACC 20262. By screening a fosmid library using kasA, thegene encoding aminotransferase, we isolated a 22 kb DNAfragment. The fragment contained seventeen complete openreading frames (ORFs); one of these ORFs, kasD, wasidentified as the gene for dNDP-glucose 4,6-dehydratase,which catalyzes the conversion of dNDP-glucose to 4-keto-6-deoxy-dNDP-glucose. The enzyme showed a broad spectrumof substrate specificity. In addition, ksR was overexpressed inE. coli BL21 and proved to be a self-resistance gene againstkasugamycin. These findings suggest that the isolated genecluster is highly likely responsible for the biosynthesis ofkasugamycin. KCI Citation Count: 7
Bibliography:G704-000169.2005.15.3.035
ISSN:1017-7825