Diversity and eotypic Structure of ECOR Collection Determined by Repetitive Extrageic Palindrominc PCR Genome Fingerpringting

• Published in: Journal of microbiology and biotechnology pp. 672 - 677
• Main Authors: Hwang, Keum-Ok, Jae-Chang Cho, Hyo-Mi Jang
• Format: Journal Article
• Language: Korean
• Published: 한국미생물·생명공학회 01.06.2005
• Subjects: 생물학
• ISSN: 1017-7825

The standard reference collection of strains for E.coli, the ECOR collection, was analyzed by a genome-basedtyping method. Seventy-one ECOR strains were subjected torepetitive extragenic palindromic PCR genome fingerprintingwith BOX primers (BOX-PCR). Using a similarity value of0.8 or more after cluster analysis of BOX-PCR fingerprintingpatterns to define the same genotypes, we identified 28 genotypesin the ECOR collection. Shannon’s entropy-based diversity indexwas 3.07, and the incident-based coverage estimator indicatedpotentially 420 genotypes among E. coli populations. Chisquaretest of goodness-of-fit showed statistically significantassociation between the genotypes defined by BOX-PCRfingerprinting and the groups previously defined by multilocusenzyme electrophoresis. This study suggests that thediversification of E. coli strains in natural populations isactively ongoing, and rep-PCR fingerprinting is a convenientand reliable method to type E. coli strains for the purposesranging from ecology to quarantine. KCI Citation Count: 1