Satety and Immunogenicity of Salmonella enterica Serovar Typhimurium IlaB in Mice

• Published in: Journal of microbiology and biotechnology pp. 609 - 615
• Main Authors: Cho, Sun-A, 이인수, 박종환, 석승혁, 이희영, 김동재, Min-Won Back, Seok-Ho Lee, Sook-Jin Hur, Sang-Ja Ban, Yoo-Kyoung Lee, 박재학
• Format: Journal Article
• Language: English
• Published: 한국미생물·생명공학회 01.06.2005
• Subjects: 생물학
• ISSN: 1017-7825

The safety and immunogenicity of an attenuatedrecombinant Salmonella vaccine strain, Salmonella entericaserovar Typhimurium llaB, was assessed. This vaccine straincould survive in low pH condition, and its ability ofintracellular survival did not differ from that of S. entericaserovar Typhimurium UK1, which is the wild-type of thevaccine strain. The mortality of the mice orally administeredwith the vaccine strain was 50% at the dose of 107 CFU. Allmice administered with 105 or 103 CFU of the vaccine strainsurvived for 3 days postinoculation (pi). However, all miceadministered with more than 103 CFU of the vaccine straindied within 3 days pi. To examine the protective effect of thevaccine strain, mice were orally immunized with 104 and 106CFU of the bacteria. Control mice were given with 0.5 ml ofphosphate buffered saline (PBS). After 8 days, the mice werechallenged with 109 CFU of S. enterica serovar TyphimuriumUK1, and mortality was examined for 5 days. The survivalrates of the mice immunized with 104 and 106 CFU of thevaccine strain were 60% and 80%, respectively, whereas allcontrol mice died within 2 days after challenging. To investigatethe immunogenicity of S. enterica serovar Typhimurium llaB,mice were orally immunized with 105 or 106 CFU ml of thevaccine strain. Five mice of each group were sacrificed at 5and 12 days after immunization, and results showed thatimmunization of the vaccine strain led to increases of IgG1,IgG2, and IgM titers against S. enterica serovar TyphimuriumUK1 in mouse sera, cytokine expressions such as IL-2, IL-4,IL-6, and IL-10 in spleen, and the lymphocyte proliferationresponse to mitogens (concanavalin A or LPS) stimulation. KCI Citation Count: 3