A Genome-wide Approach for Functional Analysis Using Rice Mutant

• Published in: Korean journal of crop science pp. 332 - 338
• Main Authors: Won Cheol Yim, 김동섭, 문준철, 장철성, 이병무
• Format: Journal Article
• Language: English
• Published: 한국작물학회 01.09.2009
• Subjects: 농학
• ISSN: 0252-9777; 2287-8432

Rapid extension of genomic database leads to the remarkable advance of functional genomics. This study proposes a novel methodology of functional analysis using 5-methyltrytophan (5 MT) mutant together with their 2-DE analysis and public microarray database. A total of 24 proteins was changed in 5 MT mutant and four remarkably different expressed proteins were identified. Among them, three spots were converted to Affymetrix probe. A total of 155 microarray samples from Gene Expression Omnibus (GEO) in NCBI was retrieved and followed by constructing gene co-expression networks over a broad range of biological issues through Self-Organising Tree Algorithm. Three co-expressing gene clusters were retrieved and each functional categorization with differential expression pattern was exhibited from 5 MT resistance mutant rice. It was indicated new co-expression networks in the mutant. This study suggests that on investigating possibility which correspond 2-DE to microarray database with their full potential. KCI Citation Count: 0