Effect of the Transformed Lactobacillus with Phytase Gene on Pig Production Performance, Nutrient Digestibility, Gut Microbes and Serum Biochemical Indexes

• Published in: Animal bioscience pp. 246 - 252
• Main Authors: J. Chang, Q.Q. Yin, R.Y. Zuo, L.Y. Chen, Q.X. Chen, X.Y. Wei, Q.F. Guan, J.W. Sun, Q.H. Zheng, X. Yang, G.Z. Ren
• Format: Journal Article
• Language: English
• Published: 아세아·태평양축산학회 01.02.2010
• Subjects: 축산학
• ISSN: 2765-0189; 2765-0235

In order to improve the availability of phytase and probiotics together, a phytase gene from Aspergillus ficuum has been expressed in Lactobacillus. In this study, the transformed Lactobacillus with phytase gene was fed to pigs to determine its effect on pig production, feed conversion and gut microbes. Forty eight, 60-day-old, castrated pigs (Duroc×Landrace×Pietrain) were assigned to 6groups, 8 pigs for each group. Group 1 was the control, group 2 was added with chlortetracycline (500 ㎎/㎏), group 3 was added with the transformed Lactobacillus (500 ㎎/㎏) with 20% (w/w) of calcium monohydrogen phosphate (CMP, CaHPO4) removed, group 4was added with the natural Lactobacillus (500 mg/kg) with 20% (w/w) of CMP removed, group 5 was added with the transformed Lactobacillus (500 ㎎/㎏) with 40% (w/w) of CMP removed, group 6 was added with phytase (500 ㎎/㎏) with 40% (w/w) of CMP removed. The results showed: i) the average daily gain (ADG) was improved in groups 2, 3 and 4 (p<0.05); ii) the diarrhea rates in the groups added with Lactobacillus were lower than in the other groups (p<0.05), in which the transformed Lactobacillus had more effect on reducing digestive disease; iii) the transformed Lactobacillus was most effective in improving the digestibilities of crude protein (CP), calcium (Ca), phosphorus (P), compared with the other groups (p<0.05); iv) Lactobacillus could increase lactic acid bacterium number and ammonia concentrations, and decrease pH values and E. coli number in pig feces (p<0.05); v) the phytase activity in the feces of pigs fed with the transformed Lactobacillus was 133.32 U/g, which was higher than in group 4 (9.58 U/g, p<0.05), and was almost the same as group 6 (135.94 U/g); vi) the transformed Lactobacillus could increase serum concentrations of IgA, triglyceride,and glutamic oxaloacetic transaminase activity (p<0.05), and had no significant effect on other serum indexes (p>0.05). KCI Citation Count: 5