The Signaling Mechanism of TGF-β_1 Induced Bovine Mammary Epithelial Cell Apoptosis

• Published in: Animal bioscience pp. 304 - 310
• Main Authors: He-Shuang Di, Li-Gang Wang, Genlin Wang, Lei Zhou, Yuanyuan Yang
• Format: Journal Article
• Language: English
• Published: 아세아·태평양축산학회 01.03.2012
• Subjects: 축산학
• ISSN: 2765-0189; 2765-0235

The present study showed that Transforming growth factor beta 1 (TGF-β_1) can induce apoptosis of bovine mammary epithelial cells. This apoptosis was also observed with phosphorylation of Smad2/3 within 0.5-2 h. Afterwards the signal transferred into the nucleus. Moreover, intracellular free Ca^(2+) concentration was significantly elevated as well as Caspase-3 activated and DNA lysised, thereby inducing the programmed cell death. This signaling pathway of TGF-β_1 was blocked by SB-431542 (10^(-2) μM) via inhibiting ALK-5 kinase activity, which thus reversed the anti-proliferation and apoptosis effect of TGF-β_1 in mammary epithelial cells. These results indicated that TGF-β_1 induced apoptosis of bovine mammary epithelial cells through the ALK-5-Smad2/3 pathway, which plays an important role in inhibiting survival of mammary epithelial cells. Moreover, intracellular Ca^(2+) also played a critical role in TGF-β_1-induced cell apoptosis. KCI Citation Count: 6