Comparison of Spectrophotometric, HPLC and Chemiluminescence Methods for 3-Nitrotyrosine and Peroxynitrite Interaction

We have studied the interaction of 3-nitrotyrosine with peroxynitrite using three different methods; chemiluminescence, spectrophotometry and HPLC. Peroxynitrite-induced luminol or lucigenin chemiluminescence were significantly decreased by 3-nitrotyrosine, in concentrationdependent manners. The int...

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Bibliographic Details
Published inArchives of pharmacal research pp. 358 - 363
Main Authors Nilufer Nermin Turan, Mustafa Ark, Abdullah Tuncay Demiryurek
Format Journal Article
LanguageKorean
Published 대한약학회 01.03.2005
Subjects
약학
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Summary:We have studied the interaction of 3-nitrotyrosine with peroxynitrite using three different methods; chemiluminescence, spectrophotometry and HPLC. Peroxynitrite-induced luminol or lucigenin chemiluminescence were significantly decreased by 3-nitrotyrosine, in concentrationdependent manners. The intensity of the peroxynitrite spectrum was also markedly reduced in the presence of 3-nitrotyrosine in the spectrophometric assay. However, there was no attenuation of the 3-nitrotyrosine signal in the HPLC assay after mixing with peroxynitrite. The interaction of 3-nitrotyrosine and hypochlorous acid (HOCl) was also studied via the hemiluminescence assay, where the HOCl-induced responses were markedly inhibited by 3-nitrotyrosine. These results suggest that caution should be taken when studying the levels or interactions of 3-nitrotyrosine. KCI Citation Count: 1
Bibliography:G704-000010.2005.28.3.002
ISSN:0253-6269
1976-3786