Transcriptional regulation of Zic3 by heterodimeric AP-1(c-Jun/c-Fos) during Xenopus development

• Published in: Experimental & molecular medicine pp. 468 - 475
• Main Authors: 이승영, 이현식, 문진수, 김종일, 박재봉, 이재용, 박매자, 김재봉
• Format: Journal Article
• Language: Korean
• Published: 생화학분자생물학회 01.10.2004
• Subjects: 생화학
• ISSN: 1226-3613; 2092-6413

The heterodimeric c-Jun/c-Fos, an activator pro-tein-1 (AP-1) has ben implicated in mesoderm induction (Dong et al., 1996; Kim et al., 1998) whereas the homodimer of c-Jun was reported to be involved in neural inhibition during the early development of Xenopus embryos. During the early vertebrate development AP-1 involve-ment in the neural induction is still not clearly understod. We report here that AP-1 has a role in Zic3 expresion, a critical proneural gene and a primary regulator of neural and neural crest development (Nakata et al., 1997; Nakata et al., 1998). AP-1 was able to induce the Zic3 gene in a dose dependent manner but other homo- or hetero-dimeric proteins, such as c-Jun/c-Jun, JunD/FosB or JunD/Fra-1 were not. The inhibi-tion of AP-1 activity using morpholino antisen-Zic3 expres-sion induced by activin. In adition, co-injection of c-jun mRNA rescued the down-regulated Zic3 expression. The promoter region of isolated Zic3 genomic DNA was found to possess several consensus-binding site of AP-1. Thus, in the functional assays, AP-1 could increase promoter activity of Zic3 gene. These findings sugest that proneural gene, Zic3 may be regulated by heterodimeric AP-1(c-Jun/c-Fos) and it may have a role in activin signaling for the regulation of neural specific gene, Zic3. KCI Citation Count: 13