Involvement of JNK-initiated p53 accumulation and phosphorylation of p53 in pseudolaric acid B induced cell death

• Published in: Experimental & molecular medicine pp. 428 - 434
• Main Authors: Xianfeng Gong, Minwei Wang, Shin-ichi Tashiro, Satoshi Onodera, Takashi Ikejima
• Format: Journal Article
• Language: Korean
• Published: 생화학분자생물학회 01.08.2006
• Subjects: 생화학
• ISSN: 1226-3613; 2092-6413

A terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay was used to determine that apoptosis causes HeLa cell death induced by pseudolaric acid B. The c-Jun N-terminal kinase (JNK) inhibitor SP600125 decreased p53 protein expression during exposure to pseudolaric acid B. SP600125 decreased the phosphorylation of p53 during pseudolaric acid B exposure, indicating that JNK mediates phosphorylation of p53 during the response to pseudolaric acid B. SP600125 reversed pseudolaric acid B-induced down-regulation of phosphorylated extracellular signal-regulated pro-tein kinase (ERK), and protein kinase C (PKC) was activated by pseudolaric acid B, whereas stau-rosporine, calphostin C, and H7 partly blocked this effect. These results indicate that p53 is partially regulated by JNK in pseudolaric acid B-induced HeLa cell death and that PKC participates in pseudolaric acid B-induced HeLa cell death. KCI Citation Count: 24