Development and validation of a simultaneous HPLC with UV detection method for quantifying five bioactive compounds in diverse Lactuca sativa varieties

• Published in: 한국식품과학회지, 57(4) pp. 400 - 407
• Main Authors: Hyeon Jun Moon, Yu Jin Kwak, Hyo Seung Choi, 임순호
• Format: Journal Article
• Language: English
• Published: 한국식품과학회 01.08.2025
• Subjects: 식품과학
• ISSN: 0367-6293

This study evaluated five bioactive marker compounds—chlorogenic acid, lactucin, quercetin, scutellarin, and lactucopicrin—in three lettuce (Lactuca sativa) cultivars: ‘Cheongchima’, ‘Cheongharang I’, and ‘Cheongharang II’, using HPLC with UV detection. The method showed excellent linearity (R2>0.999), with detection limits of 0.36 to 1.51 g/mL and quantification limits between 1.09 and 4.56 g/mL. It also exhibited good precision (relative standard deviation, RSD: 0.11- 3.93%) and accuracy (RSD: 0.04-3.50%) with recovery rates of 95.06-104.28%. Among extracts, the 60% ethanol extract of ‘Cheongharang I’ exhibited the highest levels of chlorogenic acid (745.34 g/mL) and quercetin (632.08 g/mL). Lactucin was most abundant in the 0% ethanol extract of ‘Cheongharang II’, while scutellarin peaked in the 60% ethanol extract of ‘Cheongharang I’ (1,945.47 g/mL). Lactucopicrin was highest in the 100% ethanol extract of ‘Cheongharang II’ (23.49 g/ mL). These findings provide a reliable analytical basis for quality control and bioactive profiling of lettuce cultivars. KCI Citation Count: 0