Anti-obesity Effect of Protein Hydrolysate Derived from Japanese Rhinoceros Beetle (Allomyrina dichotoma) Larvae

• Published in: 농업생명환경연구, 36(4) pp. 387 - 395
• Main Authors: 이강훈, 진해영, 안태현, 심지연, 이준호, 최태윤, 박장우, 김미정, 김미혜, 김상우, 조세열
• Format: Journal Article
• Language: English
• Published: 농업생명과학연구원 01.12.2024
• Subjects: 농학
• ISSN: 2233-8322; 2508-870X
• DOI: 10.22698/jales.20240029

In this study, the anti-obesity effects of protein hydrolysates derived from Japanese rhinoceros beetle (Allomyrina dichotoma) larvae were investigated. Four different hydrolysate fractions were assessed: Flavourzyme 10 kDa, Flavourzyme 30 kDa, Protana 10 kDa, and Protana 30 kDa. Cytotoxicity assays revealed that over 80% of cells remained viable in the presence of up to 250 μg/mL hydrolysate. Among the tested samples, Flavourzyme 10 kDa (200 μg/mL) was the most effective in suppressing the expression of sterol regulatory element-binding transcription factor-1 (SREBP-1) and fatty acid synthase (FAS), key proteins involved in lipid synthesis in 3T3-L1 adipocytes. Oil Red O staining further confirmed that Flavourzyme 10 kDa (200 μg/mL) significantly inhibited lipid accumulation during adipocyte differentiation. These results suggest that the Flavourzyme 10 kDa hydrolysate at 200 μg/mL holds promise as a functional anti-obesity ingredient in pet food products. KCI Citation Count: 0