Monoclonal antibody-based enzyme-linked immunosorbent assay for quantifi cation of majonoside R2 as an authentication marker for Nngoc Linh and Lai Chau ginsengs

• Published in: Journal of ginseng research pp. 474 - 480
• Main Authors: Jiranan Chaingam, Le Van Huy, Kanta Noguchi, Poomraphie Nuntawong, Sornkanok Vimolmangkang(Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Center of Excellence in Plant-Produced Pharmaceuticals, Chulalongkorn University), Varalee Yodsurang(Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Preclinical Toxicity and Efficacy, Assessment of Medicines and Chemicals Research Unit, Chulalongkorn University), Gorawit Yusakul, Satoshi Morimoto, Seiichi Sakamoto
• Format: Journal Article
• Language: English
• Published: 고려인삼학회 01.09.2024
• Subjects: 기타의약학
• ISSN: 1226-8453; 2093-4947
• DOI: 10.1016/j.jgr.2024.05.004

Background: Recent years have witnessed increasing interest in the high amount of ocotillol-type saponin inPanax vietnamensis, particularly in relation to majonoside R2 (MR2). This unique 3%–5% MR2 content impartNgoc Linh and Lai Chau ginsengs with unique pharmacological activities. However, in the commercial domain,unauthentic species have infiltrated and significantly hindered access to the authentic, efficacious variety. Thus,suitable analytical techniques for distinguishing authentic Vietnamese ginseng species from others is becomingincreasingly crucial. Therefore, MR2 is attracting considerable attention as a target requiring effective managementmeasures. Methods: An enzyme-linked immunosorbent assay (ELISA) was developed by producing monoclonal antibodiesagainst MR2 (mAb 16E11). The method was thoroughly validated, and the potential of the immunoassay wasconfirmed by high-performance liquid chromatography with ultraviolet spectroscopy. Furthermore, ELISA wasapplied to the assessment of the MR2 concentrations of various Panax spp., including Korean, American, andJapanese ginsengs. Results and conclusions: An icELISA using mAb 16E11 exhibited linearity between 3.91 and 250 ng/mL of MR2,with detection and quantification limits of 1.53 and 2.50 46.6 ng/mL, respectively. Based on this study, thedeveloped icELISA using mAb 16E11 could be a valuable tool for analyzing MR2 level to distinguish authenticNgoc Linh and Lai Chau ginsengs from unauthentic ones. Furthermore, the analysis of the samples demonstratedthat Ngoc Linh and Lai Chau ginsengs exhibit a notably higher MR2 value than all other Panax spp. Thus, MR2might be their ideal marker compound, and various bioactivities of this species should be explored. KCI Citation Count: 1