Foeniculum vulgare essential oil nanoemulsion inhibits Fusarium oxysporum

• Published in: Journal of ginseng research pp. 236 - 244
• Main Authors: Hongyan Nie, Hongxin Liao, Jinrui Wen, Cuiqiong Ling, Liyan Zhang, Furong Xu, Xian Dong
• Format: Journal Article
• Language: English
• Published: 고려인삼학회 01.03.2024
• Subjects: 기타의약학
• ISSN: 1226-8453; 2093-4947
• DOI: 10.1016/j.jgr.2023.12.002

Background: Fusarium oxysporum (F. oxysporum) is the primary pathogenic fungus that causes Panax notoginseng(P. notoginseng) root rot disease. To control the disease, safe and efficient antifungal pesticides must currently bedeveloped. Methods: In this study, we prepared and characterized a nanoemulsion of Foeniculum vulgare essential oil (Ne-FvEO) using ultrasonic technology and evaluated its stability. Traditional Foeniculum vulgare essential oil (TFvEO)was prepared simultaneously with 1/1000 Tween-80 and 20/1000 dimethyl sulfoxide (DMSO). The effectsand inhibitory mechanism of Ne-FvEO and T-FvEO in F. oxysporum were investigated through combinedtranscriptome and metabolome analyses. Results: Results showed that the minimum inhibitory concentration (MIC) of Ne-FvEO decreased from 3.65 mg/mL to 0.35 mg/mL, and its bioavailability increased by 10-fold. The results of gas chromatography/mass spectrometry(GC/MS) showed that T-FvEO did not contain a high content of estragole compared to Foeniculumvulgare essential oil (FvEO) and Ne-FvEO. Combined metabolome and transcriptome analysis showed that bothemulsions inhibited the growth and development of F. oxysporum through the synthesis of the cell wall and cellmembrane, energy metabolism, and genetic information of F. oxysporum mycelium. Ne-FvEO also inhibited theexpression of 2-oxoglutarate dehydrogenase and isocitrate dehydrogenase and reduced the content of 2-oxoglutarate,which inhibited the germination of spores. Conclusion: Our findings suggest that Ne-FvEO effectively inhibited the growth of F. oxysporum in P. notoginseng invivo. The findings contribute to our comprehension of the antifungal mechanism of essential oils (EOs) and laythe groundwork for the creation of plant-derived antifungal medicines. KCI Citation Count: 4