Unrecorded Fungi Isolated from Fire Blightcontrolled Apple Orchard Soil in Korea

• Published in: Hangug gynnhaghoi ji pp. 491 - 504
• Main Authors: 지수영(단국대학교, 조준우, 노형진, 김민석, 김예은, 김성환
• Format: Journal Article
• Language: English
• Published: 한국균학회 01.12.2023
• Subjects: 생물학
• ISSN: 0253-651X; 2383-5249
• DOI: 10.4489/kjm.20230049

To explore fungal diversity in orchard soil where fire-blighted apple trees are buried, wecollected soil samples from apple orchards in Chungju, Korea. Fungal isolates were obtainedfrom DG18 agar and identified at the species level based on morphological features andphylogenetic analyses. The colony characteristics and microstructures were examinedusing a light microscope and a scanning electron microscope after culturing on potatodextrose agar (PDA), malt extract agar (MEA), Czapek yeast agar (CYA), and oatmeal agar(OA) The PCR-amplified products of the ITS1-5.8S-ITS2 region and 28S large subunit of thenuclear ribosomal RNA gene, as well as partial sequences of the β-tubulin, calmodulin, andtranslation elongation factor 1-α genes were sequenced and analyzed phylogenetically. Seven previously unknown fungal species were explored in Korea. All samples, includingAspergillus aureolatus, Botryotrichum atrogriseum, Dactylonectria novozelandica, Fusariumdenticulatum, Paecilomyces tabacinus, Sarcopodium tibetense and Talaromyces stollii, hadascomycetes. Herein, we report their descriptions and features. KCI Citation Count: 0