Profiling of differentially expressed proteins between fresh and frozen-thawed Duroc boar semen using Protein- Chip CM10

• Published in: Journal of animal science and technology pp. 401 - 411
• Main Authors: Yong-Min Kim, Sung-Woo Park, Mi-Jin Lee, Da-Yeon Jeon, Su-Jin Sa, Yongdae Jeong, 성하승, 최정우, Shinichi Hochi, Eun-Seok Cho, 정학재
• Format: Journal Article
• Language: English
• Published: 한국축산학회 01.03.2023
• Subjects: 축산학
• ISSN: 2672-0191; 2093-6281

Many studies have been conducted to improve technology for semen cryopreservation in pigs. However, computer-assisted analysis of sperm motility and morphology is insufficient to predict the molecular function of frozen-thawed semen. More accurate expression patterns of boar sperm proteins may be derived using the isobaric tags for relative and absolute quantification (iTRAQ) technique. In this study, the iTRAQ-labeling system was coupled with liquid chromatography tandem-mass spectrometry (LC-MS/MS) analysis to identify differentially expressed CM10-fractionated proteins between fresh and frozen-thawed boar semen. A total of 76 protein types were identified to be differentially expressed, among which 9 and 67 proteins showed higher and lower expression in frozen-thawed than in fresh sperm samples, respectively. The classified functions of these proteins included oxidative phosphorylation, mitochondrial inner membrane and matrix, and pyruvate metabolic processes, which are involved in adenosine triphosphate (ATP) synthesis; and sperm flagellum and motile cilium, which are involved in sperm tail structure. These results suggest a possible network of biomarkers associated with survival after the cryopreservation of Duroc boar semen. KCI Citation Count: 1