배양액 종류, EGF, β-ME 및 호르몬이 돼지 난자의 체외 성숙율에 미치는 영향에 관한 연구

• Published in: Journal of animal reproduction & biotechnology (Online) pp. 247 - 253
• Main Authors: 김상근, 장서희, 이만희
• Format: Journal Article
• Language: Korean
• Published: 사단법인 한국동물생명공학회 01.09.2006
• Subjects: 축산학
• ISSN: 2671-4639; 2671-4663

본 연구는 안정된 돼지 체외 성숙 난자를 얻을 목적으로 배양액의 종류 및 배양액에 EGF, β-ME, 호르몬 첨가가 돼지 난포란의 체외 성숙에 미치는 영향을 조사하였다. 난포란을 TCM-199, NCSU-23 및 PZM-3으로 48시간 배양했을 떄 체외 성숙율은 각각 22.1±0.70%, 30.6±0.70% 및 30.4±2.82%였다. TCM-199로 48시간 배양했을 때 체외 성숙율은 NCSU-23 및 PZM-3 보다 약간 낮은 체외 발생율을 나타냈다. 난포란을 25 ng/ml의 EGF를 첨가한 TCM-199, NCSU-23 및 PZM-3로 48시간 배양했을 때 체외 성숙율은 각각 46.3±2.8%, 76.6±3.1% 및 72.2±2.6%로 나타났다. 난포란의 배양 시 배양액에 25 및 50 ng/ml의 EGF를 첨가 후 48시간 배양했을 때 첨가하지 않은 군에 비해 높은 체외 성숙율을 나타냈다(p<0.05). 난포란을 NCSU-23 및 PZM-3 배양액에 25 μM/ml의 β-ME를 첨가한 후 48시간 배양했을 때 체외 성숙율은 각각 43.9±1.41%, 41.7±1.41%, 44.4±0.70%, 40.6±0.70%로 나타났다. 난포란을 25 μM/ml의 β-ME를 첨가한 NCSU-23로 48시간 배양했을 때 첨가하지 않은 군에 비해 높은 체외 성숙율을 나타냈다(p<0.05). 난포란의 배양 시 NCSU-23에 PMSG, hCG, PMSG+hCG, hCG+β-estradiol, PMSG+β-estradiol을 첨가 후 배양하였을 때 체외 성숙율은 각각 75.6%, 77.8%, 80.0%, 86.4% 및 84.8%로서 무첨가 군(64.4%)에 비해 높게 나타났다(p<0.05). This study was investigated in vitro maturation rate of oocytes cultured in maturation media with or without supplementation of EGF, β-ME and hormones. 1. When the oocytes were cultured in TCM-199, NCSU-23 and PZM-3 for 48 hrs, the maturation rates were 25.4±0.70%, 30.4±0.70% and 30.4±2.82%, respectively. The maturation rate of oocytes cultured in TCM-199 was slightly lower than cultured in NCSU-23 and PZM-3 (p<0.05). 2. The IVM rate of oocytes cultured in TCM- 199, NCSU-23 and PZM-3 supplementation with 25 ng/ml EGF for 48 hrs were 46.3± 2.8%, 76.6±3.1%, 72.2±2.6%, respectively. When the oocytes were cultured in TCM-199, NCSU- 23 and PZM-3 supplemented with 25 ng/ml for 48 hrs, the IVM rate were higher than cultured in none supplementation of EGF (p< 0.05). 3. The IVM rate of oocytes were cultured in TCM-199, NCSU-23 and PZM-3 supplementation with 25 μM/ml β-ME for 24 or 48 hrs were 35.8±0.70%, 34.5±1.41%, 35.5±1.41 %, 32.2±0.70% and 43.9±1.41%, 41.7±1.41%, 44.4±0.70%, 40.6±0.70%, respectively. When the oocytes were cultured in TCM-199, NCSU- 23 and PZM-3 supplemented with 25 μM/ml β-ME for 48 hrs, the IVM rate were higher than cultured in non supplementation higher of β-ME (p<0.05). 4. The IVM rate of oocytes cultured in TCM- 199 with supplementation of MSG, hCG, PMSG +hCG, hCG+β-estradiol, PMSG+β-estradiol for 48 hrs were 75.6%, 77.8%, 80.0%, 86.4% and 84.8%, respectively, and the IVM rate of oocytes cultured in NCSU-23 supplemented with hormones for 48 hrs higher than cultured in non supplementation of hormones (64.4%). KCI Citation Count: 0