Closed-Tube Barcoding1

Here, we present a new approach for increasing the rate and lowering the cost of identifying, cataloging, and monitoring global biodiversity. These advances, which we call Closed-Tube Barcoding, are one application of a suite of proven PCR-based technologies invented in our laboratory. Closed-Tube B...

Full description

Saved in:
Bibliographic Details
Published inGenome Vol. 59; no. 11; pp. 1049 - 1061
Main Authors Sirianni, Nicky M, Yuan, Huijun, Rice, John E, Kaufman, Ronit S, Deng, John, Fulton, Chandler, Wangh, Lawrence J
Format Journal Article
LanguageEnglish
Published NRC Research Press 2016
Subjects
Closed-Tube Barcoding
codage à barres en tube fermé (« Closed-Tube Barcoding »)
fluorescent signatures
LATE-PCR
Lights-On/Lights-Off probes
Pan-phylum probes
signatures en fluorescence
Sondes noir et blanc (« Lights-On/Lights-Off probes »)
sondes pan-spécifiques
Online AccessGet full text

Cover

More Information
Summary:Here, we present a new approach for increasing the rate and lowering the cost of identifying, cataloging, and monitoring global biodiversity. These advances, which we call Closed-Tube Barcoding, are one application of a suite of proven PCR-based technologies invented in our laboratory. Closed-Tube Barcoding builds on and aims to enhance the profoundly important efforts of the International Barcode of Life initiative. Closed-Tube Barcoding promises to be particularly useful when large numbers of small or rare specimens need to be screened and characterized at an affordable price. This approach is also well suited for automation and for use in portable devices.
ISSN:0831-2796
1480-3321
DOI:10.1139/gen-2016-0026