Analysis of subgingival microbiota and IL-1β, TNF-α and CX3CL1 levels in gingival crevicular fluid of fixed dental prostheses

• Published in: Dental Materials Journal Vol. 43; no. 2; pp. 235 - 246
• Main Authors: Mario Alberto ALARCON-SANCHEZ, Natividad CASTRO-ALARCON, Daniel SANDOVAL-GUEVARA, Mirna VAZQUEZ-VILLAMAR, Karla FERNANDEZ-ACOSTA, Mayra Yanet MENDEZ-GOMEZ, Isela PARRA-ROJAS, Norma Samanta ROMERO-CASTRO
• Format: Journal Article
• Language: Japanese
• Published: Japanese Society for Dental Materials and Devices 2024
• ISSN: 0287-4547

Prosthetic biomaterials can affect the composition of the subgingival microbiota and consequently the production of proinflammatory cytokines, causing damage to the periodontium. A total of 40 patients were divided into two groups: 20 with monolithic zirconia (MZ) prostheses and 20 with porcelain fused to metal (PFM) with nickel-chromium (Ni-Cr) alloy prostheses. Subgingival plaque and gingival crevicular fluid samples were taken. The Checkerboard technique for DNA-DNA hybridization and the enzyme-linked immunosorbent assay technique were performed. Teeth with MZ presented a lower percentage of bleeding on probing and tooth mobility compared to teeth with PFM with Ni-Cr alloy. Prosthodontic teeth harbored higher total levels of the 18 bacterial species than non-prosthodontic teeth. There was a higher prevalence of S. gordonii and V. parvula species in PFM with Ni-Cr alloy compared to MZ. There was an increase in IL-1β, TNF-α and CX3CL1 levels in PFM with Ni-Cr alloy compared to MZ. MZ is a candidate biomaterial with fewer negative effects on the periodontium, allowing for longer prostheses longevity in the mouth.