G-24 PPARgamma polymorphism and periodontitis in pregnant Japanese women

• Published in: Journal of the Japanese Society of Periodontology Vol. 52; no. 3; p. 305
• Main Authors: Noriko Sugita, Emi Hirano, Akira Kikuchi, Yasuko Shimada, Jun Sasahara, Ruriko Iwanaga, Kenichi Tanaka, Hiromasa Yoshie
• Format: Journal Article
• Language: Japanese
• Published: The Japanese Society of Periodontology 2010
• ISSN: 0385-0110

「Abstract」: Background and objective: Recent studies suggest an association between maternal periodontitis and the risk of preterm birth, though it remains controversial. Peroxisome proliferator-activated receptor-gamma (PPARgamma) is a nuclear hormone receptor and inhibits the transcription of pro-inflammatory mediators. Genetic polymorphism PPARgamma Pro12A1a(rs 1801282) was reported to reduce the ability above. In this study, we tested whether the PPARgamma Pro12A1a was associated with periodontitis and/or preterm birth. Materials and Methods: Genomic DNA was isolated from Japanese women (term birth: 72, preterm birth: 58). The PPARgamma Pro12A1a genotype was determined by PCR-RFLP. Within 5 days after labor, clinical periodontal parameters were evaluated, and periodontopathic bacteria from the subgingival plaque were detected by PCR. Serum samples were analyzed by ELISA for IgG antibody against the periodontopathic bacteria. Results: The mean CAL (P=0.012), percentages of the sites with CAL ≧ 4mm (P=0.023), the mean PD (P=0.031), and the mean GI (P=0.037) in the women with PPARgamma Pro12A1a were significantly higher than those with the PPARgamma Pro12Pro. However, there were no differences between PPARgamma Pro12A1a genotypes in the bacterial counts or in serum IgG antibody levels against bacteria. In addition, there was no significant association between preterm birth and periodontitis. Conclusion: Our study suggested that the PPARgamma Pro12A1a may represent a genetic susceptibility factor for the clinical measurement of periodontitis in pregnant Japanese women.