W11-01 Mutant mouse library now elucidates the genome function bridging monogenic and polygenic traits

Mouse genetics is one of the driving forces to elucidate genome function. For monogenic functions, KO-first-conditional-ready ES cell lines are now available to most of the genes (Nature 2011). For polygenic functions, various systems, e.g., Collaborative Cross and Consomic Strains, have been develo...

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Bibliographic Details
Published inGenes & Genetic Systems Vol. 86; no. 6; p. 398
Main Authors GONDO Yoichi, FUKUMURA Ryutaro, MURATA Takuya, MAKINO Shigeru
Format Journal Article
LanguageJapanese
Published The Genetics Society of Japan 2011
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Summary:Mouse genetics is one of the driving forces to elucidate genome function. For monogenic functions, KO-first-conditional-ready ES cell lines are now available to most of the genes (Nature 2011). For polygenic functions, various systems, e.g., Collaborative Cross and Consomic Strains, have been developed to analyze millions of SNPs. To bridge the monogenic and polygenic analyses, the RIKEN mutant mouse library is now available to the research community. We archived ～10,000 G1 mice by ENU mutagenesis, in which each G1 carries thousands of base substitutions. We have been providing allelic series of point mutations to any target genes. Users used to conduct many generations of backcross to analyze the function in a uniform background. During the backcross, any modifiers and gene-to-gene interactions, if any, were discarded on purpose. We now propose to conduct the intercross immediately so that the all the possible traits, monogenic and polygenic, appear in the phenotype analysis. We now provide another information of genomewide mutations by the next-generation sequencer to users. This new system enables to reveal the function of the target gene together with any modifiers/epestasis.
ISSN:1341-7568