2E-03 Transposition efficiency and stability of KP element in Drosophila melanogaster

• Published in: Genes & Genetic Systems Vol. 85; no. 6; p. 433
• Main Authors: UCHIYAMA Ryo, NAKAZAWA Hitomi, MIYATAKE Kazushi, YAMAMOTO Aki, INOMATA Nobuyuki, ITOH Masanobu
• Format: Journal Article
• Language: Japanese
• Published: The Genetics Society of Japan 2010
• ISSN: 1341-7568; 1880-5779

Transposable P element in Drosophila melanogaster can be classified into two types; one is the full-size P (FP) element, and the other is internally-deleted defective elements, which cannot transposition autonomously. The KP element, one of the defective elements, is currently predominant in natural populations as well as FP element. In order to elucidate this wide spreading of KP elements, we compared its transposition efficiency and stability with those of FP element. A plasmid vector pFwK containing each of FP and KP elements was induced into 2343 embryos of w1118; SM6CyO/Sp; TM3Ubx/SbΔ2-3. We found 21 FP and 89 KP elements insertions in 208 individuals by PCR, implying that KP element transpose more frequently than FP element (χ2=44.5, p<0.01). We subsequently established P-insertion lines from the transgenic flies to follow decaying of P element for generations. Small size P element occurred de novo in the lines having FP element insertions, and almost all of such novel elements suppose to be derived from FP element even if KP elements co-existed (p<0.008). These results suggest that a high transposition efficiency and stability of KP element.