2C-02 Stop codon independent translation termination promoted by E.coli ArfA

Ribosome recycling is facilitated by release factor (RF) which recognizes specific translational stop codon. In the absence of stop codon, ribosome stalls at the end of mRNA to form nonproductive complex. Such a nonproductive complex is known to be dissolved by SsrA RNA-mediated trans-translation. R...

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Bibliographic Details
Published inGenes & Genetic Systems Vol. 85; no. 6; p. 414
Main Authors CHADANI Yuhei, ONO Katsuhiko, ABO Tatsuhiko
Format Journal Article
LanguageJapanese
Published The Genetics Society of Japan 2010
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Summary:Ribosome recycling is facilitated by release factor (RF) which recognizes specific translational stop codon. In the absence of stop codon, ribosome stalls at the end of mRNA to form nonproductive complex. Such a nonproductive complex is known to be dissolved by SsrA RNA-mediated trans-translation. Recently, we have identified ArfA (alternative ribosome-rescue factor A) which rescues stalled ribosome by different mechanism than trans-translation. ArfA protein interacts with ribosome and promotes alternative ribosome rescue when overproduced. ArfA-mediated ribosome rescue causes the degradation of non-stop mRNA, but nascent peptide is not degraded. Further in vitro analysis suggested that ArfA-mediated ribosome rescue requires cofactor(s). Synthetic lethality of ssrA arfA double mutation suggests the importance of ribosome recycling for bacterial growth. SsrA and ArfA would compensate their function each other to maintain the translational activity for cellular viability.
ISSN:1341-7568